Evaluation of phytochemical, antioxidant, cytotoxicity and in vitro antibacterial activity of aqueous extract of Ganoderma lucidum cultivated in Bangladeshi habitat

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Authors :

Md. Shihabul Islam, Md. Sifat Rahi, Habiba Khan Koli, Israt Jerin, Salek Ahmed Sajib, Kazi Md. Faisal Hoque and Md. Abu Reza*

Author Address :

Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology,
3rd science building, level# 4, University of Rajshahi, Rajshahi-6205, Bangladesh.
*For correspondence E-mail: reza.gen@ru.ac.bd

Abstract :

Ayurvedic treatment is one of the most ancient treatment systems against various diseases in Indian subcontinent and the western countries have also begun to take this alternative natural therapy as their major treatment system. The medicine of ayurvedic treatment came from various plants or plant parts. Ganoderma lucidum is one of fungus that has been extensively used as various therapeutic agents and contains approximately 400 different bioactive compounds which have been reported to have a number of pharmacological effects. But the biochemical composition of G. lucidium can be changed from its native origin. The biochemical composition, antioxidant activity, cytotoxic activity and antibacterial activity of the G. lucidium cultivated in Bangladesh were detected in this experiment. The phytochemical screening of aqueous extract of G. lucidium was determined using standard methods, the antioxidant activity was evaluated by DPPH free radical scavenging assay, the cytotoxic effect was conducted by brine shrimp lethality assay and the antibacterial activity was done using disc diffusion assay. The primary phytochemical screening of G. lucidium revealed that the extracts contain proteins, carbohydrates, glycosides, saponins, terpenoids, phenolic compounds. The result indicated that G. lucidium has good antioxidant activity and showed IC50 value as 89.05±3.59 g/mL. The aqueous extract of G. lucidium showed medium cytotoxic effect and LC50 value was calculated as 142.49±5.31 g/mL. About 40% and 60% of used bacterial strains were shown intermediate and resistance respectively after overnight incubation where Amoxicillin was used as control antibiotics. Hence, G. lucidium contains major therapeutic and antibacterial agents for potential pharmaceutical applications.

Keywords :

Antibacterial, Antioxidant, Cytotoxic effect, Ganoderma lucidum, Phytochemical,
Pharmacological effects.

DOI :

Article Info :

Received : February 02, 2018; Accepted : March 23, 2018.

 

   

 

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