Mythili Saravanan, P Senthilkumar, K Kalimuthu, G Rajalakshmi and Chinnadurai Vajjiram
Author Address :
1PG and Research Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore - 641028. 2Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – 641018. *For correspondence E-mail: email@example.com
A highly stable, cost-effective, efficient and successful protocol for indirect regeneration of a medicinal plant Turnera subulata through the leaf segment by using Murashige and Skoog (MS) nutrient medium supplemented with varied concentrations and combinations of growth hormones was established. The maximum organogenic callus initiation frequency (92%) was observed in MS medium with BAP and IAA at 12.32 and 1.8 μM/L respectively. The highest shoot initiation rate was observed in MS medium with BAP and IAA at 2.22+0.90 μM/L. The maximum shoot multiplication (82 %) was noticed on the MS medium enriched with BAP and IAA at (2.22 and 0.90μM/L) respectively. The high amount of multiple shoot (4 shoots/explant) was observed on the same combination of growth regulators. The in-vitro regenerated shoots were rooted successfully on MS medium supplemented with IAA alone at 2.86 μM/L. The well-developed healthy plantlets were effectively acclimatized after successive hardening. The higher survival percentage 92% of the healthy plantlets was under greenhouse condition in the hardening medium encompassed with decomposed coir waste, garden soil and vermiculate.
Turnera subulata, Leaf, BAP, IAA,
Article Info :
Received : April 27, 2018; Accepted : June 17, 2018.